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tlr7 8l  (InvivoGen)


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    InvivoGen tlr7 8l
    Secretion of pro-inflammatory cytokines IL-6 and TNFα by B cells in response to viral TLR ligands. (A) B cells were purified from healthy controls (white, N=3) or subjects with mild SARS-CoV-2 infection (red, N=3) then stimulated in vitro overnight with 50ng/mL PMA+ 1ug/mL Ionomycin or unstimulated control (-) and levels of IL-6, IL-1B, and TNFα were measured in cell culture supernatant. (B) B cells were purified from healthy controls (white, N=3) or subjects with mild SARS-CoV-2 infection (red, N=3) then stimulated in vitro overnight with 10ug/mL anti-Ig (H+L) F(ab)’2 + 100ng/mL CD40L with either 1ug/mL TL3RL (Poly I:C), 1ug/mL <t>TLR7/8L</t> <t>(R848),</t> or 2.5ug/mL TLR9L (CpG) and secretion of IL-6 and TNFα were measured in cell culture supernatant. Statistics: paired t test or one way ANOVA, *p<0.05, **p<0.01, ***p<0.001.
    Tlr7 8l, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 2360 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tlr7+8l/pmc10449608-87-61-69?v=InvivoGen
    Average 99 stars, based on 2360 article reviews
    tlr7 8l - by Bioz Stars, 2026-07
    99/100 stars

    Images

    1) Product Images from "Activation and pro-inflammatory cytokine production by unswitched memory B cells during SARS-CoV-2 infection"

    Article Title: Activation and pro-inflammatory cytokine production by unswitched memory B cells during SARS-CoV-2 infection

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2023.1213344

    Secretion of pro-inflammatory cytokines IL-6 and TNFα by B cells in response to viral TLR ligands. (A) B cells were purified from healthy controls (white, N=3) or subjects with mild SARS-CoV-2 infection (red, N=3) then stimulated in vitro overnight with 50ng/mL PMA+ 1ug/mL Ionomycin or unstimulated control (-) and levels of IL-6, IL-1B, and TNFα were measured in cell culture supernatant. (B) B cells were purified from healthy controls (white, N=3) or subjects with mild SARS-CoV-2 infection (red, N=3) then stimulated in vitro overnight with 10ug/mL anti-Ig (H+L) F(ab)’2 + 100ng/mL CD40L with either 1ug/mL TL3RL (Poly I:C), 1ug/mL TLR7/8L (R848), or 2.5ug/mL TLR9L (CpG) and secretion of IL-6 and TNFα were measured in cell culture supernatant. Statistics: paired t test or one way ANOVA, *p<0.05, **p<0.01, ***p<0.001.
    Figure Legend Snippet: Secretion of pro-inflammatory cytokines IL-6 and TNFα by B cells in response to viral TLR ligands. (A) B cells were purified from healthy controls (white, N=3) or subjects with mild SARS-CoV-2 infection (red, N=3) then stimulated in vitro overnight with 50ng/mL PMA+ 1ug/mL Ionomycin or unstimulated control (-) and levels of IL-6, IL-1B, and TNFα were measured in cell culture supernatant. (B) B cells were purified from healthy controls (white, N=3) or subjects with mild SARS-CoV-2 infection (red, N=3) then stimulated in vitro overnight with 10ug/mL anti-Ig (H+L) F(ab)’2 + 100ng/mL CD40L with either 1ug/mL TL3RL (Poly I:C), 1ug/mL TLR7/8L (R848), or 2.5ug/mL TLR9L (CpG) and secretion of IL-6 and TNFα were measured in cell culture supernatant. Statistics: paired t test or one way ANOVA, *p<0.05, **p<0.01, ***p<0.001.

    Techniques Used: Purification, Infection, In Vitro, Control, Cell Culture

    IL-6 and TNFα production by Unswitched and Switched Memory B cells in response to viral TLR ligand stimulation. (A) Representative flow plots from a mild SARS-CoV-2 subject and quantification of IL-6+ or (B) TNFα+ unswitched or switched memory B cells from healthy controls (white, N=3) or subjects with mild SARS-CoV-2 infection (red, N=3) upon PBMC 4 hour in vitro stimulation with 10ug/mL anti-Ig (H+L) F(ab)’2 + 100ng/mL CD40L and with either 1ug/mL TL3RL (Poly I:C), 1ug/mL TLR7/8L (R848), or 2.5ug/mL TLR9L (CpG). Statistics: one way ANOVA, ns not significant, *p<0.05, **p<0.01, ***p<0.001.
    Figure Legend Snippet: IL-6 and TNFα production by Unswitched and Switched Memory B cells in response to viral TLR ligand stimulation. (A) Representative flow plots from a mild SARS-CoV-2 subject and quantification of IL-6+ or (B) TNFα+ unswitched or switched memory B cells from healthy controls (white, N=3) or subjects with mild SARS-CoV-2 infection (red, N=3) upon PBMC 4 hour in vitro stimulation with 10ug/mL anti-Ig (H+L) F(ab)’2 + 100ng/mL CD40L and with either 1ug/mL TL3RL (Poly I:C), 1ug/mL TLR7/8L (R848), or 2.5ug/mL TLR9L (CpG). Statistics: one way ANOVA, ns not significant, *p<0.05, **p<0.01, ***p<0.001.

    Techniques Used: Infection, In Vitro



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    Secretion of pro-inflammatory cytokines IL-6 and TNFα by B cells in response to viral TLR ligands. (A) B cells were purified from healthy controls (white, N=3) or subjects with mild SARS-CoV-2 infection (red, N=3) then stimulated in vitro overnight with 50ng/mL PMA+ 1ug/mL Ionomycin or unstimulated control (-) and levels of IL-6, IL-1B, and TNFα were measured in cell culture supernatant. (B) B cells were purified from healthy controls (white, N=3) or subjects with mild SARS-CoV-2 infection (red, N=3) then stimulated in vitro overnight with 10ug/mL anti-Ig (H+L) F(ab)’2 + 100ng/mL CD40L with either 1ug/mL TL3RL (Poly I:C), 1ug/mL <t>TLR7/8L</t> <t>(R848),</t> or 2.5ug/mL TLR9L (CpG) and secretion of IL-6 and TNFα were measured in cell culture supernatant. Statistics: paired t test or one way ANOVA, *p<0.05, **p<0.01, ***p<0.001.
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    Secretion of pro-inflammatory cytokines IL-6 and TNFα by B cells in response to viral TLR ligands. (A) B cells were purified from healthy controls (white, N=3) or subjects with mild SARS-CoV-2 infection (red, N=3) then stimulated in vitro overnight with 50ng/mL PMA+ 1ug/mL Ionomycin or unstimulated control (-) and levels of IL-6, IL-1B, and TNFα were measured in cell culture supernatant. (B) B cells were purified from healthy controls (white, N=3) or subjects with mild SARS-CoV-2 infection (red, N=3) then stimulated in vitro overnight with 10ug/mL anti-Ig (H+L) F(ab)’2 + 100ng/mL CD40L with either 1ug/mL TL3RL (Poly I:C), 1ug/mL <t>TLR7/8L</t> <t>(R848),</t> or 2.5ug/mL TLR9L (CpG) and secretion of IL-6 and TNFα were measured in cell culture supernatant. Statistics: paired t test or one way ANOVA, *p<0.05, **p<0.01, ***p<0.001.
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    Image Search Results


    Secretion of pro-inflammatory cytokines IL-6 and TNFα by B cells in response to viral TLR ligands. (A) B cells were purified from healthy controls (white, N=3) or subjects with mild SARS-CoV-2 infection (red, N=3) then stimulated in vitro overnight with 50ng/mL PMA+ 1ug/mL Ionomycin or unstimulated control (-) and levels of IL-6, IL-1B, and TNFα were measured in cell culture supernatant. (B) B cells were purified from healthy controls (white, N=3) or subjects with mild SARS-CoV-2 infection (red, N=3) then stimulated in vitro overnight with 10ug/mL anti-Ig (H+L) F(ab)’2 + 100ng/mL CD40L with either 1ug/mL TL3RL (Poly I:C), 1ug/mL TLR7/8L (R848), or 2.5ug/mL TLR9L (CpG) and secretion of IL-6 and TNFα were measured in cell culture supernatant. Statistics: paired t test or one way ANOVA, *p<0.05, **p<0.01, ***p<0.001.

    Journal: Frontiers in Immunology

    Article Title: Activation and pro-inflammatory cytokine production by unswitched memory B cells during SARS-CoV-2 infection

    doi: 10.3389/fimmu.2023.1213344

    Figure Lengend Snippet: Secretion of pro-inflammatory cytokines IL-6 and TNFα by B cells in response to viral TLR ligands. (A) B cells were purified from healthy controls (white, N=3) or subjects with mild SARS-CoV-2 infection (red, N=3) then stimulated in vitro overnight with 50ng/mL PMA+ 1ug/mL Ionomycin or unstimulated control (-) and levels of IL-6, IL-1B, and TNFα were measured in cell culture supernatant. (B) B cells were purified from healthy controls (white, N=3) or subjects with mild SARS-CoV-2 infection (red, N=3) then stimulated in vitro overnight with 10ug/mL anti-Ig (H+L) F(ab)’2 + 100ng/mL CD40L with either 1ug/mL TL3RL (Poly I:C), 1ug/mL TLR7/8L (R848), or 2.5ug/mL TLR9L (CpG) and secretion of IL-6 and TNFα were measured in cell culture supernatant. Statistics: paired t test or one way ANOVA, *p<0.05, **p<0.01, ***p<0.001.

    Article Snippet: Purified B cells were plated in 96 well tissue culture plates in RPMI 1640 media with 10% FBS, 1X Glutamax, and 1X Penicillin/streptomycin (Gibco) for 18 hours at 37˚C with 5% CO2 in the presence of 50ng/mL PMA (Sigma) with 1ug/mL Ionomycin (Sigma) or 10ug/mL anti-Ig (H+L) F(ab)’2 (Southern Biotech) and 100ng/mL CD40L (Biolegend) with either 1ug/mL TL3RL (Poly I:C), 1ug/mL TLR7/8L (R848), or 2.5ug/mL TLR9L (CpG) (all from InvivoGen, San Diego, CA) as indicated in the figure legends.

    Techniques: Purification, Infection, In Vitro, Control, Cell Culture

    IL-6 and TNFα production by Unswitched and Switched Memory B cells in response to viral TLR ligand stimulation. (A) Representative flow plots from a mild SARS-CoV-2 subject and quantification of IL-6+ or (B) TNFα+ unswitched or switched memory B cells from healthy controls (white, N=3) or subjects with mild SARS-CoV-2 infection (red, N=3) upon PBMC 4 hour in vitro stimulation with 10ug/mL anti-Ig (H+L) F(ab)’2 + 100ng/mL CD40L and with either 1ug/mL TL3RL (Poly I:C), 1ug/mL TLR7/8L (R848), or 2.5ug/mL TLR9L (CpG). Statistics: one way ANOVA, ns not significant, *p<0.05, **p<0.01, ***p<0.001.

    Journal: Frontiers in Immunology

    Article Title: Activation and pro-inflammatory cytokine production by unswitched memory B cells during SARS-CoV-2 infection

    doi: 10.3389/fimmu.2023.1213344

    Figure Lengend Snippet: IL-6 and TNFα production by Unswitched and Switched Memory B cells in response to viral TLR ligand stimulation. (A) Representative flow plots from a mild SARS-CoV-2 subject and quantification of IL-6+ or (B) TNFα+ unswitched or switched memory B cells from healthy controls (white, N=3) or subjects with mild SARS-CoV-2 infection (red, N=3) upon PBMC 4 hour in vitro stimulation with 10ug/mL anti-Ig (H+L) F(ab)’2 + 100ng/mL CD40L and with either 1ug/mL TL3RL (Poly I:C), 1ug/mL TLR7/8L (R848), or 2.5ug/mL TLR9L (CpG). Statistics: one way ANOVA, ns not significant, *p<0.05, **p<0.01, ***p<0.001.

    Article Snippet: Purified B cells were plated in 96 well tissue culture plates in RPMI 1640 media with 10% FBS, 1X Glutamax, and 1X Penicillin/streptomycin (Gibco) for 18 hours at 37˚C with 5% CO2 in the presence of 50ng/mL PMA (Sigma) with 1ug/mL Ionomycin (Sigma) or 10ug/mL anti-Ig (H+L) F(ab)’2 (Southern Biotech) and 100ng/mL CD40L (Biolegend) with either 1ug/mL TL3RL (Poly I:C), 1ug/mL TLR7/8L (R848), or 2.5ug/mL TLR9L (CpG) (all from InvivoGen, San Diego, CA) as indicated in the figure legends.

    Techniques: Infection, In Vitro